R.D. Kasitowati, K. Wittriansyah, A. Trianto, D.C. Pratiwi, M.A.P. Panjaitan
Sponges produce secondary metabolites that have potential resource for medicine. Sponges have anti-inflammatory and antimicrobial activity. This study aimed to determine the antifungal activities of marine sponges Fascaplysinopsis sp. and Haliclona sp. against Candida albicans and Aspergilus flavus, and to identify its bioactive compounds. The research procedure includes extraction, thin layer chromatography, open column chromatography, antifungal test, phytochemical test and GC-MS. The result of the study revealed that the ethyl acetate extracts of Fascaplysinopsis sp. and Haliclona sp. have an antifungal activity against C. albicans and A. flavus. Fascaplysinopsis sp. exhibited strong inhibitory activity (22.71±0.44 mm / 200 μg/disk) against Candida albicans and the rest of the extracts show moderate inhibitory activity (Fascaplysinopsissp. to A.flavus 10.50±0.52 mm, Haliclonasp to C.albicans and A.flavus, 10.76±0.84 mm and 8.71±0.53 mm). Phytochemical screening assays showed Haliclona sp. contained alkaloid and steroid, while GC-MS analysis showed fraction 5 extract Fascaplysinopsis sp. consisted of 39 compounds, one of them is naphthalene which is known to have an antifungal activity. © Published under licence by IOP Publishing Ltd.
Faculty of Fisheries and Marine Science, University of Brawijaya, Malang, Indonesia; Coastal Resilience and Climate Change Adaptation, University of Brawijaya, Malang, Indonesia; Fisheries Mechanical Engineering, State Polytechnic of Cilacap, Indonesia; Faculty of Fisheries and Marine Science, Diponegoro University, Semarang, Indonesia
