Nurul Isnaini, Muhammad Nur Ihsan, Sri Wahjuningsih
This study examined the effects of mangosteen peel extract (MPE) on cryopreservation and fertility of goat sperm. The semen was collected from a buck aged at 3 years old with the body weight of 60 kg. After the initial evaluation, the semen was then diluted in the Tris-egg yolk extender supplemented with MPE either at 0, 2, 4, or 6%. The results showed that sperm quality before freezing and post-thawing in all MPE treatments were decreased (p<0.05) compared to the fresh semen, but no significant difference (p>0.05) among MPE treatments on sperm quality before freezing and post-thawing. The use of 2% MPE tended to give optimum post-thawed semen quality traits. Post-thawed semen showed a positive correlation between sperm motility and viability, but they had a negative correlation with abnormality. The fertility test was conducted on 20 fertile does. The first group was inseminated with untreated frozen semen (control) (10 does), while other 10 does were inseminated with 2% MPE frozen semen. It was observed that 2% MPE resulting in a relatively higher non-return rate at 21 days than control (80% vs 70%). Overall, the inclusion of 2% mangosteen peel extract in Tris-egg yolk extender provide optimum goat semen characteristics post-thawing. The application of artificial insemination with frozen semen treated with 2% mangosteen peel extract could beneficially affect goat fertility. © 2019, Fundacion CIPAV. All rights reserved.
Department of Animal Production, Faculty of Animal Science, University of Brawijaya, Malang, 65145, Indonesia
