Mohamad Fadjar, Sri Andayani, Cucun Herlina, Fariana Banyu Kartika, Riva Dianto, Fadila Nur Aziza, Josia Fernando Sihombing, Cyntia A. W. Lumban Batu, Luki Kristinawati, Hafizh Wijdan Wadha
Amphipoda sp. is a promising live feed candidate in aquaculture due to its nutritional profile and ecological role, yet limited studies exist on its optimal diet to enhance digestive efficiency and growth. This study aims to evaluate the effect of three different diets—fermented rice bran, Artemia salina, and green mussel (Perna viridis)—on digestive enzyme activities (amylase, protease, lipase) and growth performance of Amphipoda sp. over a 12-day period. The study employed a completely randomized design (CRD) with three different feed treatments—fermented rice bran (A), Artemia (B), and green mussel (C)—each replicated three times, to measure growth rate and the activities of amylase, protease, and lipase in Amphipoda sp. Results showed that green mussel produced the highest growth rate (0.49 g/day) and final length (8.59 mm), while fermented rice bran induced the highest amylase activity (0.0055 U/mL) due to its high carbohydrate content (47.87%). In contrast, green mussel, with the highest protein content (27.72%), led to the highest protease and lipase activities (0.67 U/mL). ANOVA confirmed significant differences among treatments (F_calculated > F_table at 5% significance). These findings indicate that green mussel is the most effective diet for supporting enzymatic function and growth, while fermented rice bran offers potential as a cost-efficient feed to enhance carbohydrate digestion. TION. © 2026, Egyptian Society for the Development of Fisheries and Human Health. All rights reserved.
Department of Aquaculture, Faculty of Fisheries and Marine Science, University of Brawijaya, Indonesia; Balai Pelatihan dan Penyuluhan Perikanan Banyuwangi, Jl. Raya Situbondo No.Km 17. Bangsring, Banyuwangi, Indonesia; PSDKU Aquaculture, Department of Water Resources Management and Fisheries Science, Faculty of Fisheries and Marine Science, University of Brawijaya, Indonesia
