Amelia Nanda Vidyaningrum, Muhammad Shafry Nurwahyudin, Chairdin Dwi Nugraha, Defi Sriwahyuni, Asrullah As, Wike Andre Septian, Ari Ardiantoro, Sri Wahjuningsih, Irida Novianti, Achadiah Rachmawati, Suyadi Suyadi
Quercetin is a polyphenolic antioxidant with free-radical–scavenging, metal-chelating, and membrane-stabilizing properties that can mitigate oxidative-stress–driven deterioration of sperm function during chilled storage. This study aimed to determine the optimal quercetin dose in a Tris–egg-yolk extender for preserving Dorper ram semen quality during 72 hours of storage at 3–5 °C. Fresh ejaculates collected using an artificial vagina and meeting preset quality thresholds were diluted with Tris–egg yolk supplemented with quercetin (0, 30, 60, 90 µM). Samples were stored for 0, 24, 48, and 72 hours and evaluated for progressive motility, viability, morphological abnormalities, and plasma-membrane integrity (HOST) under a completely randomized design (n = 5 per treatment). Quercetin exhibited time-dependent effects, where storage duration significantly influenced progressive motility and plasma-membrane integrity (P < 0.01). Although the main effect of quercetin dosage was not statistically significant for motility and viability (P > 0.05), the 30 µM group descriptively yielded the highest grand-mean motility. All groups maintained ≥40% motility through 72 hours, indicating acceptable operational quality for artificial insemination. In conclusion, supplementation with 30–60 µM quercetin is recommended for short-term liquid storage. © 2026, ResearchersLinks Ltd. All rights reserved.
Faculty of Animal Science, University of Brawijaya, Jl. Veteran, Malang, 65145, Indonesia; National Research and Innovation Agency Republic of Indonesia, Jl. M.H. Thamrin No. 8, Jakarta Pusat, 10340, Indonesia; Laboratory of Animal Biotechnology, University of Brawijaya, Jl. Veteran, Malang, 65145, Indonesia
