Firdaus Muhamad, Yahya Yahya, Priambodo Ahmad Faris, Gaisani Rizqia Zuhri Arifa
Spirulina platensis contains a group of metabolomes that can inhibit glucose absorption. This study aimed to identify metabolites in the infusion and decoction of S. platensis and evaluate their ability as inhibitors of α-amylase and α-glucosidase through the in silico method. The samples were analyzed using HPLC-HRMS by untargeted method for metabolite identification while molecular docking studies were conducted using PyRx 0.8 and physicochemical properties were analyzed using SwissADME. The results showed 120 metabolites identified, with 84 from decoction and 67 from infusions. Molecular docking studies revealed that zearalenone and adenosine have a strong binding affinity for α-amylase and α-glucosidase, with an affinity energy of-8.6 kcal/mol, lower than that of acarbose. Zearalenone forms a hydrogen bond with Ile230 residue on α-amylase while adenosine forms a hydrogen bond with His332 and Arg400 residues on α-glucosidase. Analysis of physicochemical properties according to Lipinski's rule shows that both compounds meet the criteria for good bioavailability. Further molecular and in vivo dynamics studies are needed to strengthen the results of this research. © 2026, World Researchers Associations. All rights reserved.
Faculty of Fisheries and Marine Sciences, Brawijaya University, Jl. Veteran, Malang, 65145, Indonesia
