Vanda Primaditya, Fitra Arsy Nur Cory'ah, Linda Ika Puspita Ariati, Zakiah, Dyah Woro Kartiko Kusumo Wardani, Yuningsih, Dianita Primihastuti, Husnul Khotimah, Mohammad Muljohadi Ali, Wibi Riawan
Stunting is a failure of linear growth during the first two years of life. Rotenone is pesticide causes stunting. Rotenone as Endocrine Disrupting Chemicals and complex I mitochondrial inhibitor thus causes a decrease of ATP and oxidative stress. Centella asiatica is a plant that contains high antioxidants and antiinflammation. The purpose of this study is to evaluate the effect of CA ethanolic extract toward the rotenone-induced zebrafish larvae stunting model toward the expression increasing of GLUT 4 and Osteocalcin. This experimental study used zebrafish larvae as an animal model. Rotenone 12 ppb and CA extract were administered at 2-72 hpf. Observation of body length using optilab Image Raster at 3, 6, and 9 dpf, while the GLUT 4 and osteocalcin observed at 9 dpf by wholemount immunostaining. The results showed that rotenone 12.5 ppb decreased the body length of zebrafish larvae at 6 and 9 dpf significantly and more than 2 SD shorter from control. The expression of GLUT 4 and Osteocalcin in zebrafish larvae in the rotenone-induced group decreased significantly. Administration of 5 μg/mL extract increased expression of GLUT4, while the expression of osteocalcin increased by 2.5 μg/mL and 5 μg/mL. Rotenone 12.5 ppb induced stunting of zebrafish larvae. Ethanolic extract of CA protects the zebrafish larvae from stunting through increased expression of GLUT 4 and Osteocalcin. © 2020 American Institute of Physics Inc.. All rights reserved.
Master Program of Midwifery, Faculty of Medicine, Universitas Brawijaya, Malang, East Java, Indonesia; Midwifery Academy of Berlian Nusantara, Magetan, East Java, Indonesia; Poltekkes Kemenkes Mataram, Midwifery Academy, NTB, Indonesia; Midwifery Academy of Jember, Jember, East Java, Indonesia; University of Darul Ulum Jombang High Islamic Boarding School, Jombang, East Java, Indonesia; Institute of Health Science Sint Carolus, Jakarta, Indonesia; Institute of Health Science Dr. Soebandi, Jember, East Java, Indonesia; Institute of Health Science William Booth, Surabaya, East Java, Indonesia; Laboratory of Pharmacology, Faculty of Medicine, Universitas Brawijaya, Malang, East Java, Indonesia; Laboratory of Biochemistry, Faculty of Medicine, Universitas Brawijaya, Malang, East Java, Indonesia
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