Fajar Shodiq Permata, Yudit Oktanella, Dyah Kinasih Wuragil Putu Raharjo, Handayu Untari, Mifta Rizqina Amalia
PRP (Platelet-Rich Plasma) contains platelets, which are rich in proteins, including growth factors, that play an essential role in wound healing and tissue or bone regeneration. PRP could be isolated from xenografts such as goats, which are abundant in Indonesia. This study aimed to compare the effects of three anticoagulants on PRP-originated goat blood, focusing on protein concentration and identification. The goat blood sample was preserved in three anticoagulants: heparin, sodium citrate, and EDTA(Ethylenediaminetetraacetic acid). The PRPwas obtained by double-spin centrifugation and was subjected to protein extraction. The PRP protein was visualized by SDS-PAGE using a 10% separating gel and a 5% stacking gel. The gel was stained and read in the Gel DocTM EZ System Bio-Rad reader. Protein identification used a bioinformatics approach based on UniProt and NCBI databases, with MW as the criterion. The concentration results showed higher protein recovery from blood preserved in sodium citrate than from blood preserved in lithium heparin or EDTA. The SDS-PAGE results showed decreased intensity and an obscure protein band in the heparin-and EDTA-preserved samples. In conclusion, the identified PRP-originated proteins in goat blood were beneficial growth factors. © 2026, Prof (Dr.) P. R. Yadav. All rights reserved.
Faculty of Veterinary Medicine, Universitas Brawijaya, Malang, 65151, Indonesia; School of Veterinary Science, University of Queensland, Gatton, 4343, QLD, Australia
