Kiptiyah Kiptiyah, Widodo Widodo, Gatot Ciptadi, Aulanni'am Aulanni'am, Mohammad A. Widodo, Sutiman B. Sumitro
We investigated whether 10-gingerol is able to induce oxidative stress in cumulus cells. For the in-vitro research, we used a cumulus cell culture in M199, containing 10-gingerol in various concentrations (0, 12, 16, and 20μM), and detected oxidative stress through superoxide dismutase (SOD) activity and malondialdehyde (MDA) concentrations, with incubation periods of 24, 48, 72, and 96h. The obtained results were confirmed by in-silico studies. The in-vitro data revealed that SOD activity and MDA concentration increased with increasing incubation periods: SOD activity at 0μM (1.39 ± 0.24i), 12μM (16.42 ± 0.35ab), 16μM (17.28 ± 0.55ab), 20μM (17.81 ± 0.12a), with a contribution of 71.1%. MDA concentration at 0μM (17.82 ± 1.39l), 12μM (72.99 ± 0.31c), 16μM (79.77 ± 4.19b), 20μM (85.07 ± 2.57a), with a contribution of 73.1%. Based on this, the in-silico data uncovered that 10-gingerol induces oxidative stress in cumulus cells by inhibiting HTR1A functions and inactivating GSK3B and AKT-1. 10-gingerol induces oxidative stress in cumulus cells through enhancing SOD activity and MDA concentration by inhibiting HTR1A functions and inactivating GSK3B and AKT-1. © 2020 2020 Walter de Gruyter GmbH, Berlin/Boston.
Department of Biology, Maulana Malik Ibrahim Islamic State University of Malang, Malang, 65144, Indonesia; Department of Biology, Brawijaya University of Malang, Malang, Indonesia; Brawijaya University of Malang, Malang, Indonesia; Department of Chemistry, Brawijaya University of Malang, Malang, Indonesia; Biomedical Study Programme, Brawijaya University of Malang, Malang, Indonesia