Nur Masita Silviana, Sri Andarini, Diana Lyrawati, Mohammad Hidayat
Objectives: α-actinins play structural and regulatory roles in cytoskeletal organization. They form a lattice structure that secures actin in thin filaments, which generate and transmit muscle contractile forces. The morphological and biochemical characteristics of rat masseter muscles are known to change reactions to masticatory functional loads, but their effect on α-actinins remains unknown. This study aimed to determine the response of α-actinins to masticatory functional loads. Materials and Methods: Twenty-four male Wistar rats aged 3 weeks were divided randomly into 3 groups of liquid diet (LD), soft diet, and hard diet (HD). The rats were then sacrificed at the end of 8 weeks. The middle part of superficial masseter muscles was examined to investigate the masticatory effect of functional load on the mRNA expression levels of ACTN2 and ACTN3 and the protein expression levels of α-actinin-2 and α-actinin-3. Results: The mRNA expression levels of ACTN2 and ACTN3 and the protein expression levels of α-actinin-2 of the HD group were significantly higher than those of the LD group, which served as the control group. Conclusion: Masticatory functional load organizes the mRNA expression levels of ACTN2 and ACTN3 and the protein expression levels of α-actinin-2 in rat masseter muscles through stimuli during muscle physiological adaptation. © 2021, Turkish Pharmacists Association. All rights reserved.
Universitas Brawijaya Faculty of Dentistry, Department of Orthodontics, Malang, Indonesia; Universitas Brawijaya Faculty of Medicine, Department of Public Health, Malang, Indonesia; Universitas Brawijaya Faculty of Medicine, Department of Pharmacy, Malang, Indonesia; Syaiful Anwar General Hospital Faculty of Medicine, Universitas Brawijaya, Department of Orthopaedics, Malang, Indonesia
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