V.A. Winata, R.D. Kasitowati, F. Iranawati, W.S. Pranowo, A. Sartimbul
Sardinella lemuru resources are abundant, nutritious, high omega-3 and have high economic value in artisanal fisheries. Its production volume landed for over a decade, but the most significant increase was peak southeast monsoon 2019 at Canggu fishing ground. However, prior-conventional kinship assessments genetically need to be confirmed. These research objectives encompass both molecular identification and genetic relationship of Canggu S. lemuru with the others. Similar morphological samples had collected of ten individuals. Chelex 10% was used for genomic DNA extraction, CRK-CRE primer with Hotstart-50 PCR thermal profile had applied for amplification. 1% agarose electrophoresis and Sanger sequencing had examined for both quantity and quality of D-loop amplicon. Identification using the algorithm of BLASTn. Phylogenetic tree analysis uses the neighbor-joining method. The genetic identity of 10 D-loop Canggu samples was high (97.46%-98.47%) as Philippines S. lemuru (MK579633.1-MK579742.1). The phylogenetic significantly shows low barrier inter-waters from low to moderate genetic distance. Inter-waters samples are very close in intraclade, but it still has a different mutation rate in another clade. Means suggested that Canggu S. lemuru is more diverse in nucleotide base substitutions encouraging high variation. These study results provide needed information that southern translocation occurred among waters, its dispersal connection. © Published under licence by IOP Publishing Ltd.
Department of Marine Science, Faculty of Fisheries and Marine Science (FFMS), Brawijaya University, Veteran Street, East Java, Malang, 65145, Indonesia; Marine Resources Exploration and Management (MEXMA) Research Group, Ffms, Brawijaya University, Veteran Street, East Java, Malang, 65145, Indonesia; Coastal Resilience and Climate Change Adaptation (CORECT) Research Group, Ffms, Brawijaya University, Veteran Street, East Java, Malang, 65145, Indonesia; Marine and Coastal Data Laboratory (MCDL), Marine Research Center, Ministry of Marine Affairs and Fisheries (KKP), East Ancol, Jakarta, 14430, Indonesia; Department of Hydrography, Indonesian Naval Postgraduate School (STTAL), East Ancol, Jakarta, 14430, Indonesia
Research at a Glance
Register to unlock
Topics & SDG Alignment
Register to unlock
Collaboration
Register to unlock
Author Profile (Selected)
Register to unlock
References Overview
Register to unlock
Journal & Source
Register to unlock
Metadata & Integrity
Register to unlock