Muhammad Fariq Al Husni, Irisa Trianti, Eriyanto Yusnawan, Bayu Aji Pamungkas, Sastia Prama Putri, Luqman Qurata Aini
The ability of cycads to grow in harsh environments is thought to be associated with endophytic bacteria in their roots. This study aimed to characterize endophytic bacteria from Cycas rumphii and Dioon spinulosum as stress-tolerant plant growth-promoting bacteria (PGPB). Fine roots of C. rumphii and D. spinulosum were isolated using serial dilution plating and characterized through colony morphological observation, hypersensitivity, antagonistic activity, PGP activity, and abiotic stress tolerance assays. Fourteen isolates were obtained (eight from C. rumphii and six from D. spinulosum), with higher abundance and diversity in D. spinulosum (2.51 × 1010 CFU/g; H′=2.016) than C. rumphii (4.64 × 109 CFU/g; H′=1.752). All isolates were non-pathogenic; 92% fixed nitrogen, 92% solubilized phosphate, and one (DS1A) produced IAA (0.200 ppm). Four isolates showed antagonism against Xanthomonas campestris (0.40–0.67 cm inhibition) and tolerance to drought, salinity (5–10%), and moderate heat, with growth at pH 5–6. Molecular identification by 16S rRNA sequencing revealed that CR1A, CR1B, and DS1B belong to Stenotrophomonas maltophilia, whereas DS1A belongs to Bacillus sp. These findings highlight cycads as a source of endophytic PGPB candidates for supporting plant health, particularly when grown in the environmental stress condition areas. © 2026, Agriculture Faculty Brawijaya University. All rights reserved.
Plant Pathology Study Program, Faculty of Agriculture, Universitas Brawijaya, East Java, Malang, Indonesia; Department of Plant Pest and Disease, Faculty of Agriculture, Universitas Brawijaya, East Java, Malang, Indonesia; Research Centre for Food Crops, Research Organization for Agriculture and Food, National Research and Innovation Agency (BRIN), West Java, Cibinong, Indonesia; Department of Biotechnology, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Osaka, Suita, 565-0871, Japan
